PLOS ONE: Metabarcoding Analysis of Fungal Diversity in the Phyllosphere and Carposphere of Olive Olea europaea

PLOS ONE: Metabarcoding Analysis of Fungal Diversity in the Phyllosphere and Carposphere of Olive Olea europaea.



The fungal diversity associated with leaves, flowers and fruits of olive (Olea europaea) was investigated in different phenological stages (May, June, October and December) using an implemented metabarcoding approach. It consisted of the 454 pyrosequencing of the fungal ITS2 region and the subsequent phylogenetic analysis of relevant genera along with validated reference sequences. Most sequences were identified up to the species level or were associated with a restricted number of related taxa enabling supported speculations regarding their biological role. Analyses revealed a rich fungal community with 195 different OTUs. Ascomycota was the dominating phyla representing 93.6% of the total number of detected sequences followed by unidentified fungi (3.6%) and Basidiomycota (2.8%). A higher level of diversity was revealed for leaves compared to flowers and fruits. Among plant pathogens the genus Colletotrichum represented by three species (Cgodetiae synCclavatumCacutatum s.s and Ckarstii) was the most abundant on ripe fruits but it was also detected in other organs. Pseudocercospora cladosporioides was detected with a high frequency in all leaf samples and to a less extent in ripe fruits. A much lower relative frequency was revealed for Spilocaea oleagina and for other putative pathogens including Fusarium spp., Neofusicoccum spp., and Alternaria spp. Among non-pathogen taxaAureobasidium pullulans, the species complex of Cladosporium cladosporioides and Devriesia spp. were the most represented. This study highlights the existence of a complex fungal consortium including both phytopathogenic and potentially antagonistic microorganisms that can have a significant impact on olive productions.

Colletotrichum Workshop 2014

The Colletotrichum workshop 2014 was held on March 23rd, 2014 the day before the ECFG12 at the University of Seville, Spain. Here is a link to the Workshop Program. Thank you to everyone who attended.

Also, congratulations to Sayo Kodama, graduate student of Prof. Yasuyuki Kubo, Kyoto Prefectural University, Japan, for winning a poster presentation prize during the main meeting!
Serenella Sukno and Michael Thon